LOCUS pSciT-o2 3750 bp DNA linear UNA 12-MAY-2016 DEFINITION natural linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified REFERENCE 1 (bases 1 to 3750) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 17, 2026 from SnapGene 8.2.2 https://www.snapgene.com FEATURES Location/Qualifiers source 1..3750 /mol_type="genomic DNA" /organism="unspecified" misc_feature 1..3750 /label=Source:pSciT-o2 plasmid promoter 1..401 /label=promoter from pBOT1 CDS 402..2321 /codon_start=1 /transl_table=11 /product="tetracycline ribosomal protection protein" /label=tetM /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDN TLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDG VQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPN VCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYHGSAK SNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLHLRDSV RVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIE NPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQMEVI SALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGM QYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFR MLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVI LSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMF NKIT" rep_origin 2472..3060 /label=ColE1 rep-origin misc_binding 3140..3396 /label=RP4 transfer origin misc_feature 3445..3572 /label='dnaA misc_feature 3573..3741 /label=oriC region 2 misc_feature 3632..3641 /label=DnaA box ORIGIN 1 tccctttagt gagggttaat gtcgtgactg ggaaaaccct ggcggaattc ggccagtcta 61 catgtactct ttttgataaa aaattggaga ttcctttaca aatatgctct tacgtgctat 121 tatttaagtg actatttaaa aggagttaat aaatatgcgg caaggtattc ttaaataaac 181 tgtcaatttg atagcgggaa caaataatta gatgtccttt tttaggaggg cttagttttt 241 tgtacccagt ttaagaatac ctttatcatg tgattctaaa gtatccagag aatatctgta 301 tgctttgtat acctatggtt atgcataaaa atcccggtga taaaagtatt tatcactggg 361 atttttatgc ccttttgggt ttttgaatgg aggaaatcta gatgaaaatc ataaatatcg 421 gtgtattagc tcacgttgat gcaggaaaaa caacattaac tgaatcactt ttatataact 481 ctggtgcaat tactgaactt ggttcagtag ataaaggtac tactcgtact gataatacat 541 tattagaacg tcaacgtgga atcacaattc aaacaggtat cacatctttt caatgggaaa 601 atacaaaagt aaatattata gatacacctg gacacatgga tttccttgca gaagtatacc 661 gtagtctttc agtattagat ggtgctattt tacttatcag cgctaaagat ggagttcaag 721 ctcaaactcg tatcttattt cacgcattac gtaaaatggg tattccaaca attttcttta 781 taaacaaaat tgaccaaaac ggaattgatt taagtacagt ttatcaagat atcaaagaaa 841 aactttctgc tgaaatcgtt attaaacaaa aagttgaatt atacccaaac gtttgcgtaa 901 caaattttac tgaatcagaa caatgggata cagttataga aggtaatgat gatttattag 961 aaaaatacat gtcaggtaaa tcattagaag cattagaatt agaacaagaa gaaagtattc 1021 gtttccaaaa ctgttcttta ttccctttat accatggaag cgctaaaagt aacataggta 1081 ttgataactt aattgaagtt attactaaca aattttactc ttcaactcat cgtggtcctt 1141 ctgaattatg cggtaacgtt ttcaaaattg aatatacaaa aaaacgtcaa cgtttagctt 1201 atatacgtct ttatagtggt gttttacatt tacgtgatag tgttcgtgtt agtgaaaaag 1261 aaaagattaa agttacagaa atgtatactt ctattaacgg tgaattatgc aaaattgacc 1321 gtgcatattc aggtgaaatt gtaattttac aaaacgaatt tcttaaactt aatagtgtac 1381 ttggtgacac aaaactttta ccacaacgta agaaaattga aaatccacac ccattacttc 1441 aaacaacagt agaaccaagc aaacctgaac aacgtgaaat gcttttagat gctcttttag 1501 aaattagtga ctctgaccca cttttacgtt actatgtaga ttctactact catgaaatta 1561 ttctttcttt ccttggtaaa gttcaaatgg aagttatttc tgcattatta caagaaaaat 1621 atcatgttga aatcgaatta aaagaaccta ctgtaattta tatggaacgt ccattaaaaa 1681 atgctgaata tacaattcat attgaagttc caccaaatcc attttgggct tctattggtc 1741 tttctgtttc tccacttcca cttggtagcg gaatgcaata tgaaagtagc gtaagtttag 1801 gttatcttaa tcaaagtttc caaaacgcag ttatggaagg tattcgttac ggttgcgaac 1861 aaggtttata cggttggaat gttacagact gcaaaatctg ttttaagtat ggactttact 1921 attcacctgt atcaacacct gctgactttc gtatgcttgc accaattgtt ttagaacaag 1981 ttttaaagaa agctggaact gaacttttag aaccatacct ttcttttaaa atctatgcac 2041 cacaagaata cttaagtcgt gcttataacg atgcacctaa atactgtgct aatattgttg 2101 atactcaatt aaagaacaac gaagtaattt taagcggaga aattcctgca cgttgtattc 2161 aagaatatcg tagtgattta acatttttca ctaatggacg ttctgtttgc ttaactgaat 2221 taaaaggtta tcatgttact actggtgaac ctgtatgcca accacgtcgt cctaatagtc 2281 gtattgataa agttcgttat atgttcaaca aaatcacata ataatactag tagcggccgc 2341 tgcagtaata cgactcacta tagggcatac gatttaggtg acactatagt agggataaca 2401 gggtaattta gtaggtaggg ctatatggtc gttatgcgat actgatgcat aaaggccgcg 2461 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 2521 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 2581 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 2641 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 2701 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 2761 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 2821 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 2881 aagttgtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 2941 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 3001 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 3061 cgcgcaatta accctcacta aagggaacaa aagctggagc gctgcaggaa ttcgatatca 3121 agcttatcga taccgtcgac gccggccagc ctcgcagagc aggattcccg ttgagcaccg 3181 ccaggtgcga ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac 3241 ctatcctgcc cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc 3301 aaaatcctgt atatcgtgcg aaaaagcatg gatataccga aaaaatcgct ataatgaccc 3361 cgaagcaggg ttatgcagcg gaaaagcgct ggtacccagc gatgaggatg aacccactca 3421 gggacctgat gtgacgtgct cgagcagagt tcggtggcaa agaccacaca actgtgatga 3481 atagtgttaa aaaagttgaa gcacacatta gtacaaacaa agaatttaaa catttagtaa 3541 atgcaattcg tagaaaaatt gaaggaagat agcacattat tttccacgat tgttttttaa 3601 taaaaaataa tattattcaa aactttatca agttttccac attttctaca atataatact 3661 aataaataaa aaacaatatt ttaacaatgg tatataatta tttatatatt acagaatata 3721 ctaaagtaag gagtaaaaaa aatgcgatcg //