LOCUS pJG001 3764 bp DNA circular SYN 17-SEP-2026 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3764) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 17, 2026 from SnapGene 8.2.2 https://www.snapgene.com FEATURES Location/Qualifiers source 1..3764 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 163..336 /locus_tag="pmfl578" /label=pmfl578 gene 337..1839 /locus_tag="Bxb1-integrase_2StopCodonInEcoli" /label=Bxb1 integrase /label=Bxb1-integrase_2StopCodonInEcoli misc_feature 1840..1845 /locus_tag="PvuI" /label=PvuI CDS 1846..1861 /codon_start=1 /transl_table=11 /locus_tag="tetM" /product="tetracycline ribosomal protection protein" /label=tetM /translation="QQNHI" misc_feature 1868..1919 /locus_tag="Bxb1_attP1" /label=Bxb1 attP1 /label=Bxb1_attP1 variation 1893..1894 /locus_tag="dinucleotide attP1" /label=dinucleotide attP1 terminator 1926..1956 /locus_tag="Terminator rrnDT1" /label=Terminator rrnDT1 primer_bind 1962..1984 /locus_tag="New Primer(1)" /PCR_conditions="primer sequence:cttaCATATGctgctctgatgccgcatag" /label=New Primer /label=New Primer(1) misc_feature 1966..1984 /locus_tag="F_priming_site" /label=F_priming_site misc_feature 1983..2000 /locus_tag="ter(1)" /label=ter /label=ter(1) misc_feature 1985..2282 /locus_tag="Ecoli_oriC" /label=E.coli oriC /label=Ecoli_oriC misc_feature 2265..2282 /locus_tag="ter" /label=ter primer_bind complement(2283..2302) /locus_tag="New Primer" /PCR_conditions="primer sequence:tgacggactagtGTGTCGGGGCTGGCTTAAC" /label=New Primer misc_feature 2283..2301 /locus_tag="R_priming_site" /label=R_priming_site terminator 2308..2345 /locus_tag="Terminator B1006" /label=Terminator B1006 promoter 2354..2400 /locus_tag="PN25 promoter" /label=PN25 /label=PN25 promoter CDS 2428..3216 /codon_start=1 /transl_table=11 /locus_tag="aadA1" /product="streptomycin/spectinomycin adenylyltransferase" /label=aadA1 /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK" misc_feature 2922 /locus_tag="T->C pour eliminer site HindIII" /label=T->C pour eliminer site HindIII terminator 3226..3265 /locus_tag="Terminator B1002" /label=Terminator B1002 misc_feature 3266..3317 /locus_tag="Bxb1_attP2" /label=Bxb1_attP2 variation 3291..3292 /locus_tag="dinucleotide attP2" /label=dinucleotide attP2 rep_origin join(3338..3764,1..162) /locus_tag="ColE1 rep-origin" /label=ColE1 /label=ColE1 rep-origin ORIGIN 1 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 61 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 121 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aatactcagg gacctgatgt 181 gacgtggcat gcaactttaa caacatttgt aacactaatg ggaaaacaat ctaaaagaac 241 ataatttttc aaagattttt tttgaaaaat ttgttctttt ttatttaaga gtgtaaaatt 301 attaatgtat agatattaca agagaggata aaatatatgc gcgcccttgt cgtcatccgc 361 ctttctaggg ttacagacgc cactaccagc ccggagcggc agctcgagag ctgccaacaa 421 ctgtgtgccc agcggggttg agatgtggtt ggggttgcag aggaccttga cgtctcaggg 481 gcggtcgacc cctttgatcg gaagcgacgt cctaacttag ctcgatgatt ggcttttgag 541 gaacaaccgt ttgatgtcat agtagcatac cgggtggatc gcctgacacg atcaatccgt 601 catctgcagc agttggtgca ttgggccgag gaccataaga aactggtggt gtcggcgacc 661 gaagcccatt ttgacacgac tacccctttt gctgctgttg tcattgcctt gatgggaacc 721 gtcgcacaaa tggaacttga agcaatcaag gagcgtaacc gatctgcggc acactttaac 781 attcgtgccg gcaaatatag gggttcactt ccgccttggg gatacttgcc gacgcgtgtg 841 gatggtgaat ggcgcctagt tcccgatcca gtccagcggg agaggatttt ggaagtgtat 901 caccgtgtag tagataatca cgagcccctt catttggtgg cacacgatct taataggaga 961 ggggtgctgt ctccaaagga ttatttcgcg caactacaag gacgggaacc gcaaggtagg 1021 gagtggtcag caacggcttt aaaacggtca atgatcagtg aggctatgct cgggtacgca 1081 acgttgaatg gtaaaacggt tagagacgat gacggcgcgc cgttagtacg tgcggaaccg 1141 attctgacac gagaacaact agaagcctta cgcgcagaac tggtaaagac gagtagagcc 1201 aaaccagccg tcagcacccc gtcacttctg ttacgagtgt tgttttgtgc ggtttgcggg 1261 gaacctgcct acaagtttgc aggtggtggc cgtaagcatc cacgttatcg ctgtcgctct 1321 atgggctttc ctaaacattg tggaaatggt acggtcgcga tggcagagtg ggacgcattc 1381 tgcgaggaac aagttttaga tctcctcggc gatgctgaaa gattagaaaa ggtatgggtc 1441 gcagggtccg acagtgccgt agaactcgcg gaagtaaatg cggaacttgt cgacttaact 1501 tctttaattg gatccccagc atatcgtgct ggctcgccgc agcgcgaagc tctggatgct 1561 agaatagctg cgcttgctgc gcgccaggaa gaattggaag gattagaagc tagaccttcc 1621 ggatgggaat ggcgagaaac aggacaaaga ttcggcgatt ggtggagaga acaggataca 1681 gcggctaaga atacatggct aagaagcatg aatgtgcgcc tgacattcga tgttcggggc 1741 ggcctcacaa gaacaattga ttttggagac ctacaagaat atgaacaaca tttgagactg 1801 ggaagcgttg tggaaagact tcatacagga atgtcttaac gatcgcaaca aaatcacata 1861 acctagggtg gtttgtctgg tcaaccaccg cggtctcagt ggtgtacggt acaaacccag 1921 aattcaaaac aaaaggccca gtcttccgac tgagccttac atatgctgct ctgatgccgc 1981 atagtatgtt gtaactaaag atctactgtg gataactctg tcaggaagct tggatcaacc 2041 ggtagttatc caaagaacaa ctgttgttca gtttttgagt tgtgtataac ccctcattct 2101 gatcccagct tatacggtcc aggatcaccg atcattcaca gttaatgatc ctttccaggt 2161 tgttgatctt aaaagccgga tccttgttat ccacagggca gtgcgatcct aataagagat 2221 cacaatagaa cagatctcta aataaataga tcttcttttt aatactttag ttacaacata 2281 ctgttaagcc agccccgaca cactagtaaa aaaaaccccg cccctgacag ggcggggttt 2341 tttttctagt agctcataaa aaatttattt gctttcattt ttctgtataa tagattcata 2401 cgactcacta tatggaggaa atctagaatg agagaagctg ttatagcaga agtttcaaca 2461 caattatcag aagtagttgg tgtaatagaa agacatttag aacctacttt actcgctgtt 2521 catttatatg gtagtgcagt tgatggtgga ttaaaacccc acagcgatat tgatttatta 2581 gttacagtta ctgttagatt agacgaaaca actagacgtg cattaatcaa cgatttatta 2641 gaaacatcag cgagtccagg agaatcagag atattaagag cagtagaggt aacaattgtt 2701 gttcatgatg atattattcc atggagatat ccagctaaaa gagaacttca atttggagaa 2761 tggcaaagaa acgatattct agctggaata tttgaaccag caacaattga tattgactta 2821 gcaatattac ttacgaaagc tagagaacat agtgttgcat tagttggtcc tgctgctgag 2881 gaattatttg accctgttcc agagcaagat ttatttgaag ccttaaacga aactttaaca 2941 ttatggaact caccaccaga ctgggcaggt gatgaaagaa atgttgtttt aactttaagt 3001 agaatctggt atagtgctgt aacaggaaaa attgctccta aagatgtagc tgctgactgg 3061 gccatggaaa gattaccagc acaatatcaa ccagttattt tagaagcaag acaagcgtat 3121 ttaggtcagg aggatagatt agctagtaga gctgaccaat tagaagaatt tgttcattat 3181 gtaaaaggag aaattactaa agtagtaggt aaataatcgt ctagtggatc ccgcaaaaaa 3241 ccccgcttcg gcggggtttt ttcgcgtggt ttgtctggtc aaccaccgcg ctctcagtgg 3301 tgtacggtac aaacccaggt accgcgttgc tggcgttttt ccataggctc cgcccccctg 3361 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 3421 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 3481 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 3541 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 3601 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 3661 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 3721 atgtaggcgg tgctacagag ttcttgaagt tgtggcctaa ctac //