LOCUS pMflET_o4 8015 bp ds-DNA circular 12-MAY-2016 DEFINITION pMflET-o4 plasmid, complete sequence. ACCESSION VERSION SOURCE pMflET-o4 plasmid ORGANISM pMflET-o4 plasmid other sequences; artificial sequences, vectors. REFERENCE 1 (bases 1 to 8015) AUTHORS Matteau,D., Pepin,M-E., Baby,V., Gauthier,S., Arango,M. and Rodrigue,S. TITLE Development of oriC-based plasmids for Mesoplasma florum JOURNAL Submitted (20-MAY-2016) AEM, 1752 N Street N.W. Washington DC 20036, USA FEATURES Location/Qualifiers source 1..8015 /organism="pMflET-o4 plasmid" /mol_type="other DNA" /label=source:pMflET-o4 plasmid promoter 1..401 /label=promoter from pBOT1 CDS 402..2321 /codon_start=1 /transl_table=11 /product="tetracycline ribosomal protection protein" /label=tetM CDS 2346..3428 /codon_start=1 /transl_table=11 /product="Lac repressor" /label=lacI terminator 3447..3482 /label=B1002 terminator promoter 3522..3568 /label=PN25 promoter CDS 3589..4848 /codon_start=1 /transl_table=11 /product="Erythromycin esterase type II" /label=ereB terminator 4897..4991 /label=B0014 terminator rep_origin 5131..5719 /label=ColE1 rep-origin misc_binding 5803..6059 /label=RP4 transfer origin CDS complement(6102..6151) /pseudogene="unprocessed" /codon_start=1 /transl_table=4 /note="gene truncated in 3' ; 50S ribosomal protein L34 /label=rpmH' rep_origin 6152..6451 /label=oriC region 1 misc_feature 6272..6281 /label=DnaA box misc_feature 6297..6306 /label=DnaA box(1) misc_feature 6384..6393 /label=DnaA box(2) misc_feature 6397..6406 /label=DnaA box(3) CDS 6452..7783 /codon_start=1 /transl_table=4 /product="chromosome replication initiator DnaA" /label=dnaA rep_origin 7784..8015 /label=oriC region 2 misc_feature 7810..7819 /label=DnaA box(4) misc_feature 7879..7888 /label=DnaA box(5) misc_feature 7992..8001 /label=DnaA box(6) ORIGIN 1 TCCCTTTAGT GAGGGTTAAT GTCGTGACTG GGAAAACCCT GGCGGAATTC GGCCAGTCTA 61 CATGTACTCT TTTTGATAAA AAATTGGAGA TTCCTTTACA AATATGCTCT TACGTGCTAT 121 TATTTAAGTG ACTATTTAAA AGGAGTTAAT AAATATGCGG CAAGGTATTC TTAAATAAAC 181 TGTCAATTTG ATAGCGGGAA CAAATAATTA GATGTCCTTT TTTAGGAGGG CTTAGTTTTT 241 TGTACCCAGT TTAAGAATAC CTTTATCATG TGATTCTAAA GTATCCAGAG AATATCTGTA 301 TGCTTTGTAT ACCTATGGTT ATGCATAAAA ATCCCGGTGA TAAAAGTATT TATCACTGGG 361 ATTTTTATGC CCTTTTGGGT TTTTGAATGG AGGAAATCTA GATGAAAATC ATAAATATCG 421 GTGTATTAGC TCACGTTGAT GCAGGAAAAA CAACATTAAC TGAATCACTT TTATATAACT 481 CTGGTGCAAT TACTGAACTT GGTTCAGTAG ATAAAGGTAC TACTCGTACT GATAATACAT 541 TATTAGAACG TCAACGTGGA ATCACAATTC AAACAGGTAT CACATCTTTT CAATGGGAAA 601 ATACAAAAGT AAATATTATA GATACACCTG GACACATGGA TTTCCTTGCA GAAGTATACC 661 GTAGTCTTTC AGTATTAGAT GGTGCTATTT TACTTATCAG CGCTAAAGAT GGAGTTCAAG 721 CTCAAACTCG TATCTTATTT CACGCATTAC GTAAAATGGG TATTCCAACA ATTTTCTTTA 781 TAAACAAAAT TGACCAAAAC GGAATTGATT TAAGTACAGT TTATCAAGAT ATCAAAGAAA 841 AACTTTCTGC TGAAATCGTT ATTAAACAAA AAGTTGAATT ATACCCAAAC GTTTGCGTAA 901 CAAATTTTAC TGAATCAGAA CAATGGGATA CAGTTATAGA AGGTAATGAT GATTTATTAG 961 AAAAATACAT GTCAGGTAAA TCATTAGAAG CATTAGAATT AGAACAAGAA GAAAGTATTC 1021 GTTTCCAAAA CTGTTCTTTA TTCCCTTTAT ACCATGGAAG CGCTAAAAGT AACATAGGTA 1081 TTGATAACTT AATTGAAGTT ATTACTAACA AATTTTACTC TTCAACTCAT CGTGGTCCTT 1141 CTGAATTATG CGGTAACGTT TTCAAAATTG AATATACAAA AAAACGTCAA CGTTTAGCTT 1201 ATATACGTCT TTATAGTGGT GTTTTACATT TACGTGATAG TGTTCGTGTT AGTGAAAAAG 1261 AAAAGATTAA AGTTACAGAA ATGTATACTT CTATTAACGG TGAATTATGC AAAATTGACC 1321 GTGCATATTC AGGTGAAATT GTAATTTTAC AAAACGAATT TCTTAAACTT AATAGTGTAC 1381 TTGGTGACAC AAAACTTTTA CCACAACGTA AGAAAATTGA AAATCCACAC CCATTACTTC 1441 AAACAACAGT AGAACCAAGC AAACCTGAAC AACGTGAAAT GCTTTTAGAT GCTCTTTTAG 1501 AAATTAGTGA CTCTGACCCA CTTTTACGTT ACTATGTAGA TTCTACTACT CATGAAATTA 1561 TTCTTTCTTT CCTTGGTAAA GTTCAAATGG AAGTTATTTC TGCATTATTA CAAGAAAAAT 1621 ATCATGTTGA AATCGAATTA AAAGAACCTA CTGTAATTTA TATGGAACGT CCATTAAAAA 1681 ATGCTGAATA TACAATTCAT ATTGAAGTTC CACCAAATCC ATTTTGGGCT TCTATTGGTC 1741 TTTCTGTTTC TCCACTTCCA CTTGGTAGCG GAATGCAATA TGAAAGTAGC GTAAGTTTAG 1801 GTTATCTTAA TCAAAGTTTC CAAAACGCAG TTATGGAAGG TATTCGTTAC GGTTGCGAAC 1861 AAGGTTTATA CGGTTGGAAT GTTACAGACT GCAAAATCTG TTTTAAGTAT GGACTTTACT 1921 ATTCACCTGT ATCAACACCT GCTGACTTTC GTATGCTTGC ACCAATTGTT TTAGAACAAG 1981 TTTTAAAGAA AGCTGGAACT GAACTTTTAG AACCATACCT TTCTTTTAAA ATCTATGCAC 2041 CACAAGAATA CTTAAGTCGT GCTTATAACG ATGCACCTAA ATACTGTGCT AATATTGTTG 2101 ATACTCAATT AAAGAACAAC GAAGTAATTT TAAGCGGAGA AATTCCTGCA CGTTGTATTC 2161 AAGAATATCG TAGTGATTTA ACATTTTTCA CTAATGGACG TTCTGTTTGC TTAACTGAAT 2221 TAAAAGGTTA TCATGTTACT ACTGGTGAAC CTGTATGCCA ACCACGTCGT CCTAATAGTC 2281 GTATTGATAA AGTTCGTTAT ATGTTCAACA AAATCACATA ATAATACTAG TAGGAGGata 2341 aatAGatgaa accagttaca ttgtatgatg tcgctgaata tgctggagtg agttaTCAGA 2401 CAGTTTCAAG AGTTGTTAAT CAAGCATCAC ACGTTAGCGC AAAAACTAGA GAAAAAGTTG 2461 AAGCAGCAAT GGCTGAATTA AACTATATTC CAAATAGAGT TGCACAACAA TTAGCAGGAA 2521 AACAATCTTT ATTAATTGGA GTTGCAACTT CATCTCTTGC TCTACATGCT CCATCACTAA 2581 TTGTTGCAGC AATTAAATCT AGAGCAGACC AATTAGGAGC TTCTGTAGTT GTTTCAATGG 2641 TTGAAAGAAG TGGCGTTGAA GCATGTAAGG CAGCAGTGCA CAATTTATTA GCACAAAGAG 2701 TATCAGGTCT AATTATTAAT TATCCATTAG ATGACCAAGA CGCAATAGCT GTAGAAGCAG 2761 CTTGTACTAA TGTTCCAGCT CTTTTTTTAG ATGTATCAGA CCAAACACCT ATTAATTCAA 2821 TTATTTTTTC ACATGAGGAT GGAACTAGGT TAGGGGTTGA ACATTTAGTA GCATTAGGTC 2881 ATCAACAAAT TGCATTACTT GCTGGTCCAC TTAGTAGTGT TTCAGCTCGT TTAAGATTAG 2941 CAGGATGgCA TAGATATTTA ACACGTAATC AAATACAACC AATTGCAGAA AGAGAAGGTG 3001 ATTGgAGTGC AATGAGTGGA TTTCAACAAA CCATGCAAAT GTTAAATGAA GGAATAGTTC 3061 CAACTGCCAT GTTGGTTGCT AATGACCAAA TGGCATTAGG TGCAATGCGT GCAATTACTG 3121 AATCAGGTCT TAGAGTTGGA GCTGATATTT CAGTTGTTGG ATATGACGAT ACTGAAGACT 3181 CTTCATGTTA TATTCCACCT CTTACAACTA TAAAGCAAGA TTTTAGATTA TTAGGACGGA 3241 CAAGCGTTGA TAGACTTTTA CAATTATCAC AAGGGCAGGC TGTTAAAGGT AATCAATTAC 3301 TACCAGTTAG TCTTGTGAAA AGAAAAACTA CACTTGCTCC AAATACTCAA ACTGCTTCAC 3361 CTAGAGCTTT AGCTGATTCC CTAATGCAAT TAGCTAGACA AGTTTCAAGA CTAGAATCAG 3421 GCCAAtaacc atatcatagg gccctactCG CAAAAAACCC CGCTTCGGCG GGGTTTTTTC 3481 GCggattttg ggatggcGGC GCGCCgcgaa aatctcctgg TTCATAAAAA ATTTATTTGC 3541 TTTCAGGAAA TTTTTTCTGT ATAATAGAAT AGACTGGAGG AAATCTAGAT GAGATTTGAA 3601 GAATGGGTTA AAGATAAACA TATTCCATTC AAATTAAATC ACCCTGATGA CAACTATGAT 3661 GACTTTAAAC CGCTTAGGAA AATTATTGGA GATACTCGTG TTGTAGCATT AGGAGAAAAT 3721 AGCCACTTCA TTAAAGAATT CTTCTTACTT AGACATACTT TACTTAGATT CTTCATTGAG 3781 GATTTAGGAT TTACAACTTT TGCATTTGAA TTTGGTTTTG CTGAAGGACA AATTATAAAT 3841 AACTGGATAC ATGGTCAAGG TACTGATGAC GAAATTGGTC GTTTCTTAAA ACACTTTTAT 3901 TATCCAGAAG AGTTGAAAAC AACATTCTTA TGGTTAAGAG AATATAATAA AGCCGCAAAA 3961 GAGAAAATCA CATTCTTAGG GATTGATATC CCTAGAAACG GAGGTTCATA TTTACCAAAC 4021 ATGGAAATAG TGCATGACTT CTTTAGAACT GCTGATAAAG AAGCTCTACA TATAATTGAT 4081 GACGCTTTTA ATATTGCTAA GAAGATTGAT TACTTTTCAA CTTCACAAGC AGCTTTAAAT 4141 TTACATGAAT TAACAGATAG TGAGAAATGT AGATTAACAA GTCAATTAGC AAGAGTAAAA 4201 GTTAGATTAG AAGCTATGGC ACCAATCCAC ATTGAGAAAT ATGGAATTGA TAAATATGAA 4261 ACAATATTAC ATTATGCAAA TGGAATGATT TATTTAGATT ATAATATTCA AGCAATGAGT 4321 GGTTTTATTA GTGGTGGAGG TATGCAAGGA GATATGGGAG CAAAAGATAA ATATATGGCT 4381 GATTCTGTTT TATGGCATCT TAAGAATCCA CAATCAGAAC AGAAAGTTAT AGTTGTAGCT 4441 CATAATGCAC ATATTCAGAA AACACCTATT CTATATGATG GATTTCTAAG TTGTTTACCT 4501 ATGGGACAAA GATTGAAGAA TGCTATAGGT GATGATTATA TGTCTTTAGG TATAACATCT 4561 TATTCTGGAC ATACTGCTGC ATTATATCCA GAAGTTGATA CTAAATACGG TTTTAGAGTT 4621 GATAATTTTC AATTGCAAGA ACCTAATGAA GGCTCAGTAG AAAAGGCAAT TAGCGGTTGT 4681 GGAGTTACAA ACTCTTTTGT ATTCTTTAGA AATATACCAG AAGATTTGCA ATCAATTCCA 4741 AATATGATTA GATTTGATTC AATTTACATG AAAGCTGAAT TAGAGAAAGC GTTTGATGGT 4801 ATATTTCAAA TAGAGAAATC ATCAGTTTCA GAAGTTGTTT ACGAATAACT CGAGagcgga 4861 gatttcgggt atcaatgggt accaccttac ctggactcac actggctcac cttcgggtgg 4921 gcctttctgc gtttatatac tagagagaga atataaaaag ccagattata aatccggctt 4981 ttttattatt tGCGGCCGCT GCAGTAATAC GACTCACTAT AGGGCATACG ATTTAGGTGA 5041 CACTATAGTA GGGATAACAG GGTAATTTAG taggtagggc tatatggtcg ttatgcgata 5101 ctgatgcata aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 5161 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 5221 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 5281 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 5341 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 5401 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 5461 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 5521 cggtgctaca gagttcttga agttgtggcc taactacggc tacactagaa gaacagtatt 5581 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 5641 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 5701 cagaaaaaaa ggatctcaac gcgcaattaa ccctcactaa agggaacaaa agctggagcg 5761 ctgcaggaat tcgatatcaa gctagcttat cgataccgtc gacgccggcc agcctcgcag 5821 agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc 5881 gctcgcgggt gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg 5941 aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagc atggatatac 6001 cgaaaaaatc gctataatga ccccgaagca gggttatgca gcggaaaagc gctggtaccc 6061 aGCGATGAGG ATGAACCCAC TCAGGGACCT GATGTGACGT GCCGTGAACA CGAGCGTGTT 6121 TAGTTTTTGA TGGTTGTCAA GTTCTTTTCA Ttgtattcaa accccctttt aaagttgata 6181 attcttgtta attataatat aagtgctgga aaaagtatag ttttttattg ttatttttaa 6241 gaaaatttat ccattgtgtt tttatatgaa ctttttcaac attttttata accttttgtg 6301 gaaaagcgga aaaaagatat ataaatacta aactattgct ttttagtgtt attcattatg 6361 tttatttctt tttaaaatat ttttttatcc acacattgtg gaagactttt attattatgt 6421 gttttaattt attttatgaa ggggaattaa tatggaaaca aaagcgcttt gagaaaaatt 6481 aattaataag ttaaagaagg aaaaattaat agaccaagac attattgaag aacatattgt 6541 tacttcagaa ttaattaaaa tttcaaatac agagttcgta atccttgtta gaagtaatct 6601 tggtgtaact attttaaatg aatttaaaga agtatttgtg tacgaattta aataagtttt 6661 aaacagttat gtgtctgttg attttttaac taaagaaatt tttgagaaaa atacaaaaaa 6721 agagaataaa aaagaaccca taaatactgt tttatctgaa aatgcactta cttttgagaa 6781 ttttatagtt gggtcaagta ataaacaagc taatttagct gctaaaaatg ttgttgcaaa 6841 ccctggtatg agttttaatc ctttatttat ttatggagat tcaggactgg gtaaaacaca 6901 tttattacaa gctattaaaa atcaagcaga attaaatgga aaaaaagttt tatatttaac 6961 atctgaagaa tttacaaaaa gaattgttaa tgctttaaac aaaggtgatt taagtgaaat 7021 agaagaatta aaaactgaga taaattctaa tgaattcttt attttagatg atgttcaatt 7081 tttaagtaaa aaagataaaa caaacgagtt tttctttaac attattaaca attttactga 7141 aaatggtaaa caattagttt tttctagtga taagactcct gaattgttaa atggttttga 7201 taaaagaatg ataactagat ttaattcagg tttatcaaca cctattaatg ctttagatat 7261 tccaactgcc aaattgataa ttgaagctga aattaaaaaa caaggtttaa aacaaaaaat 7321 taaagaagat gccgttgttt acttagctca aaactttagc gatgatgtta gaaaaattaa 7381 aggtttagtt aatagattac ttttctttgg tattcaaaac gatttaggtc acataattga 7441 tttggaagat gttattgact tatttaaaga tacaccttca gctaatttag gattattaaa 7501 tgttaaaaaa attaaagaag ttgttgctaa aaaatatgat gttactataa aagccatcga 7561 tggtaaagca agaacaactg ctataaaaaa tgctagacat ctttctatgt attttgcaaa 7621 aataatttta aaccatacat caactcaaat tggtgcagaa tttggtggaa gagaccatag 7681 tacagtttta agtgctattt cgcgtattga aaaattaata tataaagaaa aagaattcaa 7741 aaaaatagtt gagtctttaa aaaacgaaat aataggaaaa tagatgtaaa ttcgcatcta 7801 ttttttttgt gtggaaaaga aattaaactt gttgaataaa aagtcattta aacctttatt 7861 taccgttgtt ttatgagtgt tttccacata tcaactgcat tataaatatt acttatataa 7921 agaataatat aatatataaa agcatgcgta tttcttttgt ttttatttta taaatatgag 7981 ataatatata ttgtaaataa gaggtgttat atgag //